Journal: Magnetic Resonance Imaging

Article Title: Repeatability and reproducibility of longitudinal relaxation rate in 12 small-animal MRI systems

doi: 10.1016/j.mri.2019.03.008

Figure Lengend Snippet: Equipment used. All equipment was manufactured by Bruker (Rheinstetten, Germany) using Avance (Av) spectrometers and ParaVision (PV) acquisition and analysis software except: (a) Magnet from the companies which formerly traded as Varian, Magnex or Agilent; (b) Transmitter-Receiver from Rapid MR International, Columbus OH USA or Rimpar, Germany.

Article Snippet: K , 9.4 a , Biospec 94/30 Av III , 670 (B-GA 12S HP) , Quadrature 400 MHz (87) , PV 6.0.1.

Techniques: Software

Journal: eLife

Article Title: Functional exploration of heterotrimeric kinesin-II in IFT and ciliary length control in Chlamydomonas

doi: 10.7554/eLife.58868

Figure Lengend Snippet: ( A ) Strategy for expression and purification CrKinesin-II. ( B ) Coomassie-stained SDS-PAGE of purified FLA10/FLA8 from an MBP column (left) and KAP from a Ni column (right). ( C ) Elution profiles of CrKinesin-II from gel filtration. . ( D ) Analysis of purified CrKinesin-II by SDS-PAGE and immunoblotting. Elution fractions of CrKinesin-II from gel filtration were separated on a 10% SDS-PAGE followed by coomassie blue staining (top) or by immunoblotting with the indicated antibodies (bottom). An asterisk indicates the fraction used for motility assay. ( E–F ) FLA8’-MBP/FLA8-RFP/KAP-GFP-His and FLA10’-RFP/FLA10-MBP/KAP-GFP-His were expressed respectively in Sf9 cells followed by purification using MBP and Ni affinity columns. The purified products were analyzed by SDS-PAGE followed by coomassie blue staining ( E ) or immunoblotting ( F ).

Article Snippet: Cell line ( S. frugiperda ) , Sf9 , Expression Systems , Sf9 , .

Techniques: Expressing, Purification, Staining, SDS Page, Filtration, Western Blot, Motility Assay

Journal: eLife

Article Title: Functional exploration of heterotrimeric kinesin-II in IFT and ciliary length control in Chlamydomonas

doi: 10.7554/eLife.58868

Figure Lengend Snippet: ( A–C ) Expression and purification of recombinant HsKinesin-II. Strategy of expression and purification ( A ). Purified kinesin-II was subjected to SDS-PAGE analysis followed by coomassie blue staining ( B ) or immunoblotting with antibodies against KIF3A, KIF3B or KAP3 ( C ). ( D ) Purification of chimeric CrKinesin-II with motor domains of HsKinesin-II. The motor domains of recombinant CrKinesin-II as shown in was replaced with one or two motor domains of HsKinesin-II as indicated. The resulting constructs KIF3A’-MBP/FLA8-RFP/KAP-GFP-His, KIF3B’-RFP/FLA10-MBP/KAP-GFP-His and KIF3A’-MBP/KIF3B’-RFP/KAP-GFP-His were expressed respectively in Sf9 cells followed by purification using MBP and Ni affinity columns consecutively. The purified products were analyzed by SDS-PAGE followed by commassie blue staining.

Article Snippet: Cell line ( S. frugiperda ) , Sf9 , Expression Systems , Sf9 , .

Techniques: Expressing, Purification, Recombinant, SDS Page, Staining, Western Blot, Construct

Journal: eLife

Article Title: Functional exploration of heterotrimeric kinesin-II in IFT and ciliary length control in Chlamydomonas

doi: 10.7554/eLife.58868

Figure Lengend Snippet:

Article Snippet: Cell line ( S. frugiperda ) , Sf9 , Expression Systems , Sf9 , .

Techniques: Transformation Assay, Expressing, Transfection, Construct, Software